. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Open the catalog to page 2. Prepare your i3/i3x instrument for shipment. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. 1. Itemploysa. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellEvery SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. Price from $9. The dual auto injector module. Ease of use Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. 10. Immunofluorescence of In Vitro LPS-Stimulated Neutrophils. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. com The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Bioz Stars score: 98/100, based on 1 PubMed citations. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). Keywords: htrf spectramax detection cartridge, spectramax i3, spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf. Asterisks indicate the dilution of assay control that should be minimally detectable for each kit. . Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Products : SoftMax Pro GxP Software, SoftMax Pro Software. SpectraMax i3x Register or Sign In. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyThe SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. Restart the system. Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes—many of which catalyze the covalent regulatory. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringfluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiterA microplate reader that can detect two or more applications is considered a multi-mode plate reader. each new lot of plates, to ensure that the center of each well is read. Spectramax I3x, supplied by Danaher Inc, used in various techniques. GloMax Discover System. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. At each time point, the samples were shaken and fluorescence was measured for SEP (488-nm excitation and 530-nm emission) and. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. Actual shake speed is based on the microplate format. 99 to $1999. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. Download SoftMax® Pro 7. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. AlphaScreen. . . Negative samples contained only MycoAlert Assay Buffer. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. Contact Local Team. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. • Removing the transport locks from the PMT shutters and drawers. ü; ü. Bioz Stars score: 98/100, based on 1 PubMed citations. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Spectranet customer portalThe SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. . pH-Xtra Glycolysis Assay - Agilent. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. Supported Windows Operating System: See release notes. The plate was then read on the SpectraMax iD5, SpectraMax i3x, and SpectraMax M5e readers using the settings in Table 1. 58% for the detection module and 2. o For example: Ex: 485/Em 525/Cutoff 515 4. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. 因为是导师第一任学生，没有师兄师姐，加上本人悟性也差，小小pcr花了半年时间才终于有点样子。因为经历过，所以懂得，故而分享之，希望能给到屏幕前的你一点点帮助! 如有失误，欢迎指正~最后，不要忘了一键三联哟，谢谢大家！, 视频播放量 6293、弹幕量 2、点赞数 122、投硬币枚数 55、收藏. 22 μ m fi ltration. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. It allows you to explore cellular pathways and protein activation and expression in one system. SpectraMax iD3 and iD5 Register or Sign In. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. 0´ 104 8. Description 1. Nucleic Acid and Protein Quantitation. . SpectraMaxi3Multi. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. Spectranet customer portalThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. Tip: MCF-7 cells have a polygonal morphology with irregular dimensions, and they have a habit of clumping into large aggregates. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The calculated LOQ values were 0. A 1:2 dilution series of BSA was prepared in PBS, starting at 100 mg/mL, in LoBind microcentrifuge tubes. Expand your lab’s capabilities to include flash applications such as dual luciferase and. Sensitive TRF detection of two simple, mix-and-measure assay workflows. . For more sample throughput, the system canMicroplates were sealed to reduce evaporation (adhesive PCR plate seal; Thermo Fisher Scientific, AB0558) and placed in a microplate reader (Molecular Devices, SpectraMax i3x) for 8 h at 30 °C. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. 12911151) 0. 1 and above: Compatible with only with 64-bit OS. It allows you to explore cellular pathways and protein activation and expression in one system. Multimode Reader Comparison Chart. . Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 635. . ; SoftMax® Pro 7. Fractional occupancy Corrected BRET ratio NanoBRET. 2 Note Section Patch:1) Select the correct plate and the wells that you want to measure. Automated for ease of use. Molecular Devices Software. Methods. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The StemoniX microBrain 3D platform is a homogenous 3D neural spheroid system in 96 and 384-well format. This process should take about 3 - 5 minutes. 1. Get a front row view of your cells. Whether you are looking for an intuitive, easy-to-use reader for. Serial number 33 900 01-3387. . 9000-0756. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. Introduction Inflammation, the body’s reaction to infection, irritation, or other injury, is accompanied by increased endothelial chemokine. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. Our absorbance plate readers feature our PathCheck Sensor. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Research Article AntioxidantActivity,PolyphenolicContent,andFT-NIR AnalysisofDifferentAspiliaafricanaMedicinalPlantTissues DenisOkello ,1,2,3YuseongChung,1HyoseonKim. It allows you to explore cellular . Results for SpectraMax i3x reader with NanoBRET Detection Module (blue) and on-board luminescence detection mode (orange). Controls containing all reaction. SpectraMax Microplate Readers. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. The experiments were performed in triplicates and the spectral reading was recorded using Spectramax i3x microplate reader. +44 118 944 8000. Description 1. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. SpectraMax® i3x Multi-Mode Detection Platform; SpectraMax® i3 Multi-Mode Detection Platform; SpectraMax® ID3 Multimode Microplate reader; SpectraMax® ID5 Multimode Microplate reader; SpectraMax® Paradigm® Multi-Mode Microplate Reader; SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. Other technologies such as imaging. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. 6% for the on-board optics (monochromators). Downloads. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. com. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. ZERO BIAS - scores, article reviews, protocol conditions and more. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. 3) At the bottom of the strip, click on "Show. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence,. Western blot assay 5 x 105 cells were seeded in 24-well plates in 500 μl growth medium and stimulated with novel compounds at 10μM. 100 fmol 1536-well (8 µL) 0. using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. g. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. Find regional support. SpectraMax® i3x. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. モジュールを追加してアップグレードすることにより、ウェスタンブロットやイメージング、試薬インジェクタを用いた高速カイネティック測定を行う. SpectraMax® i3x. SpectraMax i3x Multi-Mode Detection Platform. 003 fmol 0. 306 plate air cal. ; The top 3 importers of Spectramax are India with 365. We would like to show you a description here but the site won’t allow us. 0. Nuclear Receptor. The dynamic range of the assay is about 3. SpectraMax® Microplate Readers and SoftMax® Pro Software. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. This update is applicable for users of the SpectraMax® i3x Multi-Mode Detection Platform and SpectraMax® Paradigm® Multi-Mode Microplate Reader with the ScanLater Western Blot (WB) Detection Cartridge and the SpectraMax® iD5 Multi-Mode Microplate Reader: SMP 7. Spectramax i3 manualSpectramax i3 manual Microplate spectramax i3x molecular biocompareSpectramax i3. 2. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. It has a cooled PMT, a no-stain cell analysis algorithm, and a field-upgradeable imaging cytometer. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 100 fmol 384-well (75 µL) 0. Ribonuclease (RNase) R digestion assay For identifying the stabilization of circVRK1, 2 μg isolated RNA from HBMECs was digested by RNase R (3 U/μg; RNR07250, Geneseed, Guangzhou,. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. compound concentration, and an IC 50 value of 21. 99. pathways and protein activation and expression in one system. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. After 2 days of incubation, LUC and REN activities were measured using a SpectraMax ® i3x Multi-Mode detection platform (Molecular Devices, USA) with a Dual-Luciferase Reporter Assay Kit (Promega. 3. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. Antigen Quantitation Protocol. It allows you to explore cellular pathways and protein activation and expression in one system. ü; ü. 蛍光 発光 ELISA. Overview of Western Blot Cartridge. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. Figure 1. Background subtraction was not necessary. 1. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate. HeLa cells were plated at 15,000 cells per well in 100 µL. 더 많은. user upgradeable. . The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. GM3000 Register or Sign In. . GloMax ® Navigator System. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. 660-665 Eskdale Road. Figure 1. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. We would like to show you a description here but the site won’t allow us. . The SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. (SpectraMax i3/i3x) CatchPoint SimpleStep ELISA (SpectraMax Paradigm) SpectraDrop. . In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Price from $9. Published Dec 16, 2020 | Updated Dec 16, 2020. Modularity does provide some measure of forward vision into upcoming or future experimental needs. D 1 1. Expanded dynamic range The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Simplified workflow: Add cellular imaging assays in a small footprint. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Absorbance Fluorescence (top/bottom read) Luminescence (top read) Time-Resolved Fluorescence (top read). The Curcumin binding to p53Y220C was also measured using the emission maxima of Curcumin at 495 nm after. Thetypeof luciferase(Renilla. Materials and methodsSpectraMax i3x Multi-Mode Microplate Reader. We would like to show you a description here but the site won’t allow us. Introduction. Prepare your iD series instrument for shipment. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. 0´ 105 dsD NA (p g/ wel ) R F UFluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). SPECTRAMAX i3X, INJECTOR MODULE CARTRIDGE model number 0200-7029. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax® i3x injector module with SmartInject® Technology ensuring equal mixing across the plate for high-precision experiments. Remove the transport bracket and store the retaining screws in the bag that held the 3. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. Figure 2-23: Bottle holder To install the bottle holder: 1. Description 1. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The chamber. . One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. The calculated LOQ values were 0. We would like to show you a description here but the site won’t allow us. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析 We would like to show you a description here but the site won’t allow us. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. i3x. 在这里，我们展示了如何使用SpectraMax®i3x和 SpectraMax®iD5多功能微孔板读板机应用HTRF技术进行 Gq-偶联受体的分析。G蛋白偶联受体的分析主要通过检测 cAMP和IP3这两条信号转导通路调控介导的细胞内钙离子水 平的升高。Cisbio公司的IP-One Gq试剂盒通过检. Product Details. Register to download your free eBook today. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viabilitySoftMax® Pro 7. SpectraMax Paradigm Cisbio HTRF Detection Cartridge User Guide 5008539 A 5 Laser Light Laser Specifications The SpectraMax® Paradigm® Multi-Mode Detection Platform is rated a Class 1 Laser Product because it houses one or more Laser Modules whereas the laser light is not accessible. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. 4. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Find our technical support information along with contact details. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application. Using optimized settings for the Transcreener FP assays presented in this application note, the SpectraMax i3x. 314 low lamp level warning. The SpectraMax i3x reader offers a modular design allowing the addition of FP detection to the instrument in just minutes. For wavelengths in the UV range above 220 nm,. . +1 800-635-5577. SpectraMax M5. . 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . . The user guide also covers the features, specifications, and software of the reader, as well as the installation and. For each kit, the reader was able to detect the lowest dilution as positive. Enzyme Activation Analysis;. 1321N1, a human astrocytoma cell line. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Find our technical support information along with contact details. Genscript cPass SARS-CoV-2 Neutralization Antibody Detection. Structured Review. Background Parallax,Columns 2. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. ü; Instrument Diagnostics Mode (for SpectraMax® i3x, SpectraMax® iD3, SpectraMax® iD5, SpectraMax® Paradigm)Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. Microplate Readers SpectraMax. More Product Information. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. 1 1 10 100 e e e e e e eSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 030 fmol. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. 3: The cuvette chamber. Published Dec 16, 2020 | Updated Oct 10, 2023. ValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. 58% for the detection module and 2. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. 2 Parallel Line Analysis Conc log [ng/ML] RLU. 9 x 10-15. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. SpectraMax i3x and Paradigm readers require the FP-FLUO. 1. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. iD5 reader (the SpectraMax iD3 reader is also suitable for these assays, as it has the same onboard fluorescence performance as the SpectraMax iD5 reader), SpectraMax i3x, SpectraMax M4 or M5, and FilterMax F5 Multi-Mode Microplate Readers. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataSpectraMax i3x Multi-Mode Microplate Reader. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析Spectramax i3x fluorescence plate reader to measure fluo-rescence resonance energy transfer (FRET) signals from cell lines expressing CFP-YFP FRET sensors for glucose and ATP, as these sensors give large changes in FRET ratio in response to appropriate conditions, and are expressed in cells at high levels. . Replicate values were averaged and imported into Design-Expert 8. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 305 reference level saturation. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Quantiferon-TB Gold in Tube Kit. Catalog excerpts. 312 gain calibration failed. Figure 4. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. NORTH AMERICA. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks. Download PDF. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. MitoXpress-Xtra Oxygen Consumption Assay - Agilent. no. 312 gain calibration failed. Find regional support. Download PDF. Structured Review. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Ease of use. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. SpectraMax® ABS and ABS Plus reader support; ü. (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. TaqMan SNP. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. Subsequently, 100 μL of the substrate solution (2 mM of p-nitrophenyl butyrate in dimethylformamide) was added into the mixture, and incubated at 37°C for 15 min. Supported Windows Operating System: See release notes.